Transgenic Core Facility

2024

• Hsu HC, Hsu SP, Hsu FY, Chang M and Chen JA (2024) LncRNA Litchi is a regulator for harmonizing maturity and resilient functionality in spinal motor neurons. iScience Volume 27, Issue 3, 15 March 2024, 109207.
• Huang TN, Shih YT, Yen TL and Hsueh Y (2024) Vcp overexpression and leucine supplementation extend lifespan and ameliorate neuromuscular junction phenotypes of a SOD1G93A-ALS mouse model. Human Molecular Genetics; ddae022. https://doi.org/10.1093/hmg/ddae022

2023

• Tsai YY, Shen CL, D Dhananjaya, Tsai CY and Tarn WY (2023) Activation of TrkB signaling mitigates cerebellar anomalies caused by Rbm4-Bdnf deficiency. Commun Biol 6, 910. https://doi.org/10.1038/s42003-023-05294-z
• Wang JP, Hung CH, Liou YH, Liu CC, Yeh KH, Wang KY, Lai ZS, Hsu TC, Lee TL, Shyu YC, Hsiao PW, Chen LY, Chuang TJ, Yu CH Albert, Liao NS and Shen CK James (2023) Hematopoietic Transfer of the Anti-Cancer and Lifespan-Extending Capabilities of A Genetically Engineered Blood System. Stem Cells and Regenerative Medicine , https://doi.org/10.7554/eLife.88275.1
• Lin YS, Chang YC, Chao TL, Tsai YM, Jhuang SJ, Ho YH, Lai TY, Liu YL, Chen CY, Tsai CY, Hsueh YP, Chang SY, Chuang TH, Lee CY, Hsu LC. (2023) The Src-ZNFRF1 axis controls TLR3 trafficking and interferon responses to limit lung barrier damage. J. of Experimental Medicine, 220(8), e20220727.
• Kulkarni R, Kasani SK, Tsai CY, Tung SY, Yeh KH, Yu Albert CH and Chang W (2023) FAM21 is critical for TLR2-CLEC4E-mediated dendritic cell function against Candida albicans. Life Sci Alliance. 30;6(4): e202201414. doi: 10.26508/lsa.202201414.
• Liau ES, Jin S, Chen YC, Liu WS, Calon M, Nedelec S, Nie Q and Chen JA . Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons. Nat Commun 14, 46 (2023). https://doi.org/10.1038/s41467-022-35574-x

2022

• Yen TL , Huang TN , Lin MH , Hsu TT, Lu MH, Shih PY, Ellegood J, Lerch J and Hsueh YP (2022) Sex bias in social deficits, neural circuits and nutrient demand in Cttnbp2 autism models. Brain, awac429, https://doi.org/10.1093/brain/awac429
• Huang HY, Liao HY, Chen X, Wang SW, Cheng CW, Shahed-Al-Mahmud M, Liu YM, Mohapatra A, Chen TH, Lo Jennifer M., Wu YM, Ma HH, Chang YH, Tsai HY, Chou YC, Hsueh YP, Tsai CY, Huang PY, Chang SY, Chao TL, Kao HC, Tsai YM, Chen YH, Wu CY, Jan JT, Cheng TJ Rachel, Lin KI , Ma C and Wong CH (2022) Vaccination with SARS-CoV-2 spike protein lacking glycan shields elicits enhanced protective responses in animal models. Sci Transl Med ;14 (639):eabm0899. doi: 10.1126/scitranslmed.abm0899.

2021

• Hung CH, Lee TL, Huang Anna YS, Yang KC, Shyu YC, Wen SC, Lu MJ, Yuan S and Shen CK James (2021) A Positive Regulatory Feedback Loop between EKLF/KLF1 and TAL1/SCL Sustaining the Erythropoiesis. Int J Mol Sci. 22(15): 8024. doi: 10.3390/ijms22158024.
• Lee YH, Tsai YS, PhD, Chang CC Ho CC Shih HM, Chen HM, Lai HL, Lee CW, Lee YC, Liao YC, Yang UC, Cheng TH, Chern Y, and BW (2021) A PIAS1 Protective Variant S510G Delays polyQ Disease Onset by Modifying Protein Homeostasis. Movement Disorders, Dec 23, 2021. doi: 10.1002/mds.28896.
• Chen CY, Hung YF, Tsai CY, Shih YC, Chou TF, Lai MZ, Wang TF and Hsueh YP (2021) Transcriptomic Analysis and C-Terminal Epitope Tagging Reveal Differential Processing and Signaling of Endogenous TLR3 and TLR7. Front. Immunol. 12:686060. doi: 10.3389/fimmu.2021.686060
• Tsai CY, Chen CY, Jan JT, Chou YC, Chang ML, Lu LA, Huang PY, Chu Mandy FC, Hsu TT and Hsueh YP (2021) Sex-biased response to and brain cell infection by SARS-CoV-2 in a highly susceptible human ACE2 transgenic model. bioRxiv May 2. https://doi.org/10.1101/2021.05.04.441029
• Chiang SY, Wu HC, Lin SY, Chen HY, Wang CF, Yeh NH, Shih JH, Huang YS, Kuo HC, Chou SJ* and Chen RH*(2021) Usp11 controls cortical neurogenesis and neuronal migration through Sox11 stabilization. Science Advances 7(7): eabc6093
• Li CJ, Liau ES, Lee YH, Huang YZ, Liu Z, Willems A, Garside V, McGlinn E, Chen* JA and Hong* T (2021) MicroRNA governs bistable cell differentiation and lineage segregation via a noncanonical feedback. Mol Syst Biol 17:e9945
• Chang SH, Su YC, Chang M and Chen JA (2021) MicroRNAs mediate precise control of spinal interneuron populations to exert 2 delicate sensory-to-motor outputs. eLife 10: e63768
• Huang TN, Shih YT, Lin SC and Hsueh YP (2021) Social behaviors and contextual memory of Vcp mutant mice are sensitive to nutrition and can be ameliorated by amino acid supplementation. iScience 24 (1),101949

2020

• Hung CH, Wang KY, Liou YH, Wang JP, Huang Anna YS, Lee TL, Jiang ST, Liao NS, Shyu YC and Shen CK James (2020) Negative Regulation of the Differentiation of Flk2− CD34− LSK Hematopoietic Stem Cells by EKLF/KLF1. Int J Mol Sci. 21(22): 8448
• Hu HT, Huang TN and Hsueh YP (2020) KLHL17/Actinfilin, a brain-specific gene associated with infantile spasms and autism, regulates dendritic spine enlargement. J Biomed Sci 27, 103.
• Shih PY, Hsieh BY, Tsai CY, Lo CA, Chen BE and Hsueh YP (2020) Autism-linked mutations of CTTNBP2 reduce social interaction and impair dendritic spine formation via diverse mechanisms. Acta Neuropathol Commun 8, 185
• Hung CH, Lee CH, Tsai MH, Chen CH, Lin HF, Hsu CY, Lai CL and Chen CC (2020) Activation of acid-sensing ion channel 3 by lysophosphatidylcholine 16:0 mediates psychological stress-induced fibromyalgia-like pain. Annals of the Rheumatic Diseases doi: 10.1136/annrheumdis-2020-218329
• Shih YT, Huang TN, Hu HT, Yen TL and Hsueh YP (2020) Vcp Overexpression and Leucine Supplementation Increase Protein Synthesis and Improve Fear Memory and Social Interaction of Nf1 Mutant Mice. Cell Reports 31, 107835
• Shih PY, Hsieh BY, Lin MH, Huang TN, Tsai CY, Pong WL, Lee SP and Hsueh YP (2020) CTTNBP2 Controls Synaptic Expression of Zinc-Related Autism-Associated Proteins and Regulates Synapse Formation and Autism-like Behaviors. Cell Reports 31, 107700 
• Lee M, Liu YC, Chen Chen, Lu CH, Lu ST, Huang TN, Hsu MT, Hsueh YP and Cheng PL ( 2020) Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. J Cell Biol 219 (2): e201903033.

2019

• Tung YT, Peng KC, Chen YC, Yen YP, Chang M, Thams S and Chen JA (2019) Mir-17~92 Confers Motor Neuron Subtype Differential Resistance to ALS-Associated Degeneration. Cell Stem Cell 25:1.
• Chuang TW, Lu CC, Su CH, Wu PY, Easwvaran S, Lee CC, Kuo HC, Hung KY, Lee KM, Tsai CY and Tarn WY (2019) The RNA Processing Factor Y14 Participates in DNA Damage Response and Repair. iScience 13: 402

2018

• Chen HF, Hsu CM and Huang YS (2018) CPEB2‐dependent translation of long 3’‐UTR Ucp1 mRNA promotes thermogenesis in brown adipose tissue.EMBO J 37: e99071
• Hung YF, Chen CY, Shih YC, Liu HY, Huang C-M and Hsueh YP (2018) Endosomal TLR3, TLR7, and TLR8 control neuronal morphology through different transcriptional programs. Journal of cell biology 217(8): 2727
• Chang S, Bok P, Tsai CY, Sun CP, Liu H, Deussing JM and Huang GJ. (2018) NPTX2 is a key component in the regulation of anxiety. Neuropsychopharmacology 43(9):1943

2017

• Yang P, Wang Y, Hoang D, Tinkham M, Patel A, Sun MA, Wolf G, Baker M, Chien HC, Lai KY, Cheng X, Shen CK James and Macfarlan TS (2017) A placental growth factor is silenced in mouse embryos by the zinc finger protein ZFP568. Science 356(6339):757.
• Wu YS, Chen CC, Chien CL, Lai HL, Jiang ST, Chen YC, Lai LP, Hsiao WF, Chen WP and Chern Yijuang (2017) The type VI adenylyl cyclase protects cardiomyocytes from β-adrenergic stress by a PKA/STAT3-dependent pathway. Journal of Biomedical Science 24:68.
• Li CJ, Hong T, Tung YT, Yen YP, Hsu HC, Lu YL, Chang M, Nie Q and Chen JA (2017) MicroRNA filters Hox temporal transcription noise to confer boundary formation in the spinal cord. Nature Communications 8:14685.

2016

• 張美鈴，周姿妤，蔡青宴。CRISPR/Cas9 基因剔除快手；秀傳醫學雜誌 2016. 15(3/4):138-149.
• Liou AT, Wu SY, Liao CC, Chang YS, Chang CS and Shih C (2016) A new animal model containing human SCARB2 and lacking stat-1 is highly susceptible to EV71. Scientific Reports 6:31151.
• Cheng IC, Chen BC, Shuai HH, Chien FC, Chen P and Hsieh TS (2016) Wuho Is a New Member in Maintaining Genome Stability through its Interaction with Flap Endonuclease 1. PLoSBiol 14 (1): e1002349.
• Lo HF, Tsai CY, Chen CP, Wang LJ, Lee YS, Chen CY, Liang CT, Cheong ML and Chen HW (2016) Association of dysfunctional synapse defective 1 (SYDE1) with restricted fetal growth – SYDE1 regulates placental cell migration and invasion. The Journal of Pathology. 2016. 241(3): 324–336.
• Lin SH, Cheng YR, Banks RW, Min MY, Bewick GS and Chen CC (2016) Evidence for the involvement of ASIC3 in sensory mechanotransduction in proprioceptors. Nature Communications 7:11460.
• Shih YT and Hsueh YP (2016) VCP and ATL1 regulate endoplasmic reticulum to control protein synthesis for dendritic spine formation. Nature Communications 7:11020.

2015

• Huang PL, Hou MS, Wang SW, Chang CL, Liou YH, Liao NS (2015) Skeletal muscle interleukin 15 promotes CD8(+) T-cell function and autoimmune myositis. Skelet Muscle 5:33.
• Lu CS, Hung AF, Lin CJ, Chen JB, Chen C , Shiung YY, Tsai CY and Chang TW (2015) Generating allergen-specific human IgEs for immunoassays by employing human epsilon gene knockin mice. Allergy 70:384-390.
• Tsai CY, Tsai CY, Arnold SJ and Huang GJ. (2015) Ablation of hippocampal neurogenesis in mice impairs the response to stress during the dark cycle. Nature Communincation. 6:8373.
• Sun CK, Leu S, Hsu SY, Zhen YY, Chang LT, Tsai CY, Chen YL, Chen YT, Tsai TH4, Lee FY, Sheu JJ, Chang HW and Yip HK (2015) Mixed serum-deprived and normal adipose-derived mesenchymal stem cells against acute lung ischemia-reperfusion injury in rats. American Journal of Translational Research. 7(2):209-31.
• Lin SH, Chien YC, Chiang WW, Liu YZ, Lien CC and Chen CC (2015) Genetic mapping of ASIC4 and contrasting phenotype to ASIC1a in modulating innate fear and anxiety. Eur J Neurosci, 41: 1553-1568.

2014

• Lin YH, Tsai CY, Huang LW, Seow KM, Hwang JL and Tzeng CR (2014) Reduced uterine receptivity for mouse embryos developed from in-vitro matured oocytes. Journal of assisted reproduction and genetics 31:1713-1718.
• Liou YH, Wang SW, Chang CL, Huang PL, Hou MS, Lai YG, Lee GA, Jiang ST, Tsai CY, and Liao NS (2014). Adipocyte IL-15 regulates local and systemic NK cell development. Journal of immunology 193:1747-1758.
• Huang GJ, Edwards A, Tsai CY, Lee YS, Peng L, Era T, Hirabayashi Y, Tsai CY, Nishikawa SI, Iwakura Y, Chen SJ and Flint J (2014) Ectopic cerebellar cell migration causes maldevelopment of Purkinje cells and abnormal motor behaviour in Cxcr4 null mice. PLoS one 9(2): e86471-480.
• Leu S, Lu HI, Sun CK, Sheu JJ, Chen YL, Tsai TH, Yeh KH, Chai HT, Chua S, Tsai CY, Changf HW, Leeb FY and Yipa HK (2014) Retention of endothelial progenitor cells in bone marrow in a murine model of endogenous tissue plasminogen activator (tPA) deficiency in response to critical limb ischemia. International journal of cardiology 170:394-405.

2013

• Tsai LY, Chang YW, Lin PY, Chou HJ, Liu TJ, Lee PT, Huang WH, Tsou YL and Huang YS (2013) CPEB4 Knockout Mice Exhibit Normal Hippocampus-Related Synaptic Plasticity and Memory. PLOS ONE 8(12): e84978.
• Chao HW, Tsai LY, Lu YL, Lin PY, Huang WH, Chou HJ, Lu WH, Lin HC, Lee PT and Huang YS. (2013) Deletion of CPEB3 Enhances Hippocampus-Dependent Memory via Increasing Expressions of PSD95 and NMDA Receptors. Journal of Neurosciences 33(43):17008-17022.

• Chiang YJ, Ho KC., Sun, C.T., Chiu, J.J., Lee, F.J., Liao, F., Yang-Yen, H.F., and Yen, J.J. (2013). CBAP functions as a novel component in chemokine-induced ZAP70-mediated T-cell adhesion and migration. PloS one 8, e61761.
• Chien Y, Cheng WC, Wu MR, Jiang ST, Shen CK, and Chung, B.C. (2013) Misregulated progesterone secretion and impaired pregnancy in CYP11A1 transgenic mice. Biology of reproduction 89:91.
• Liu HC, Ting CH, Wen HL, Tsai LK, Hsieh-Li HM, Li H, and Sue LC. (2013). Sodium vanadate combined with L-ascorbic acid delays disease progression, enhances motor performance, and ameliorates muscle atrophy and weakness in mice with spinal muscular atrophy. BMC medicine 11: 38.
• Yen CH, Tsai TH, Leu S, Chen YL, Chang LT, Chai HT, Chung SY, Chua S, Tsai CY, Chang HW, Ko SF, Sun CK and Yip HK. (2013) Sildenafil improves long-term effect of endothelial progenitor cell-based treatment for monocrotaline-induced rat pulmonary arterial hypertension. Cytotherapy 15:209-223.
• Yip HK, Chang YC, Wallace CG, Chang LT, Tsai TH, Chen YL, Chang HW, Leu S, Zhen YY, Tsai CY, Yeh KH, Sun CK and Yen CH. (2013) Melatonin treatment improves adipose-derived mesenchymal stem cell therapy for acute lung ischemia-reperfusion injury. Journal of Pineal Research 54:207-221.

2012

• Chien HC, Wang HY, Su YN, Lai KY, Lu LC, Chen PC, Tsai SF, Wu CI, Hsieh WS and Shen CK James (2012) Targeted Disruption in Mice of a Neural Stem Cell-Maintaining, KRAB-Zn Finger-Encoding Gene That Has Rapidly Evolved in the Human Lineage. PLoS One 7(10): e47481.
• Wu LS, Cheng WC and Shen CK (2012) Targeted depletion of TDP-43 expression in the spinal cord motor neurons leads to the development of amyotrophic lateral sclerosis-like phenotypes in mice. The Journal of biological chemistry 287:27335-27344.
• Wang HT, Liu CF, Tsai TH, Chen YL, Chang HW, Tsai CY, Leu S, Zhen YY, Chia HT, Chung SY, Chua S, Yen CH and Yip HK (2012) Effect of obesity reduction on preservation of heart function and attenuation of left ventricular remodeling, oxidative stress and inflammation in obese mice. Journal of Translational Medicine 10(1):145.
• Sheu JJ, Lin KC, Tsai CY, Tsai TH, Leu S, Yen CH, Chen YL, Chang HW, Sun CK, Chua S, Yang JL and Yip HK (2012) Combination of cilostazol and clopidogrel attenuates rat critical limb ischemia. Journal Of Translational Medicine 10:164.
• Wang HT, Liu CF, Tsai TH, Chen YL, Chang HW, Tsai CY, Leu S, Zhen YY, Chai HT, Chung SY, Chua S, Yen CH and Yip HK (2012) Effect of obesity reduction on preservation of heart function and attenuation of left ventricular remodeling, oxidative stress and inflammation in obese mice. Journal Of Translational Medicine 10(1):145.

2011

• Hsu RJ, Hsiao KM, Lin MJ, Li CY, Wang LC and Chen LK (2011) Long Tract of Untranslated CAG Repeats Is Deleterious in Transgenic Mice. PLoS ONE 6 (1): e16417.
• Chang CL, Lai YG, Hou MS, Huang PL, and Liao NS (2011) IL-15Ralpha of radiation-resistant cells is necessary and sufficient for thymic invariant NKT cell survival and functional maturation. Journal of immunology 187:1235-1242.
• Chen CY, Lin CW, Chang CY, Jiang ST and Hsueh YP (2011) Sarm1, a negative regulator of innate immunity, interacts with syndecan-2 and regulates neuronal morphology. The Journal of cell biology 193: 769-784.
• Chung WC, Huang TN and Hsueh YP (2011). Targeted deletion of CASK-interacting nucleosome assembly protein causes higher locomotor and exploratory activities. Neuro-Signals 19:128-141.
• Lee GA, Liou YH, Wang SW, Ko KL, Jiang ST and Liao NS (2011) Different NK cell developmental events require different levels of IL-15 trans-presentation. Journal of immunology 187:1212-1221.
• Mamasuew K, Hofmann N, Kretzschmann V, Biel M, Yang RB, Breer H and Fleischer J (2011) Chemo- and thermosensory responsiveness of Grueneberg ganglion neurons relies on cyclic guanosine monophosphate signaling elements. Neuro-Signals 19:198-209.

2010

• Tsai KJ, Yang CH, Fang YH, Cho KH, Chien WL, Wang WT, Wu TW, Lin CP, Fu WM and Shen CK (2010) Elevated expression of TDP-43 in the forebrain of mice is sufficient to cause neurological and pathological phenotypes mimicking FTLD-U. The Journal of experimental medicine 207: 1661-1673.
• Chien CL, Wu YS, Lai HL, Chen YH, Jiang ST, Shih CM, Lin SS, Chang C and Chern Y (2010) Impaired water reabsorption in mice deficient in the type VI adenylyl cyclase (AC6). FEBS letters 584:2883-2890.
• Wu LS, Cheng WC, Hou SC, Yan YT, Jiang ST and Shen CK (2010) TDP-43, a neuro-pathosignature factor, is essential for early mouse embryogenesis. Genesis 48, 56-62.

2009

• Hsiao HW, Liu WH, Wang CJ, Lo YH, Wu YH, Jiang ST and Lai MZ (2009) Deltex1 is a target of the transcription factor NFAT that promotes T cell anergy. Immunity 31:72-83.
• Jiang ST, Chiou YY, Wang E, Chien YL, Ho HH, Tsai FJ, Lin CY, Tsai SP, and Li H (2009) Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse. Human molecular genetics 18:1566-1577.
• Tsai KJ, Yang CH, Lee PC, Wang WT, Chiu MJ and Shen CK (2009) Asymmetric expression patterns of brain transthyretin in normal mice and a transgenic mouse model of Alzheimer's disease. Neuroscience 159:638-646.
• Yang CY, Lin NH, Lee JM, Huang CY, Min HJ, Yen JJ, Liao NS and Yang-Yen HF (2009) Promoter knock-in mutations reveal a role of Mcl-1 in thymocyte-positive selection and tissue or cell lineage-specific regulation of Mcl-1 expression. Journal of immunology 182:2959-2968.

2008

• Bose JK, Wang IF, Hung L, Tarn WY and Shen CK (2008) TDP-43 overexpression enhances exon 7 inclusion during the survival of motor neuron pre-mRNA splicing. The Journal of biological chemistry 283:28852-28859.
• Jiang ST, Chiou YY, Wang E, Lin HK, Lee SP, Lu HY, Wang CK, Tang MJ, and Li H (2008) Targeted disruption of Nphp1 causes male infertility due to defects in the later steps of sperm morphogenesis in mice. Human molecular genetics 17:3368-3379.
• Lin H, Cheng CF, Hou HH, Lian WS, Chao YC, Ciou YY, Djoko B, Tsai MT, Cheng CJ and Yang RB (2008) Disruption of guanylyl cyclase-G protects against acute renal injury. Journal of the American Society of Nephrology 19(2): 339-348.
• Tu CF, Yan YT, Wu SY, Djoko B, Tsai MT, Cheng CJ and Yang RB (2008) Domain and functional analysis of a novel platelet-endothelial cell surface protein, SCUBE1. The Journal of biological chemistry 283:12478-12488.

2007

• Chen SH, Wu PS, Chou CH, Yan YT, Liu H, Weng SY and Yang-Yen HF (2007) A knockout mouse approach reveals that TCTP functions as an essential factor for cell proliferation and survival in a tissue- or cell type-specific manner. Molecular biology of the cell 18:2525-2532.
• Yang HY, Cheng CF, Djoko B, Lian WS, Tu CF, Tsai MT, Chen YH, Chen CC, Cheng CJ and Yang RB (2007) Transgenic overexpression of the secreted, extracellular EGF-CUB domain-containing protein SCUBE3 induces cardiac hypertrophy in mice. Cardiovascular research 75:139-147.