Apparatus of FACS Core

Cell Analyzers

  • LSRII-12P(2004)
  • LSRII-18P(2002)
  • FACSCalibur (1997)
  • FACScan (1990)
  • LSR2 photo

    LSRⅡ
    Laser Detector LP mirror BP Filter(MM) Fluorochrome
    Octagon
    (488-nm blue laser)
    A 755 LP 780 / 60 PE-Cy7
    B 635 LP 695 /40 PE-Cy5
    C 550 LP 575 / 26 PE , PI
    D 505 LP 530 / 30 FITC , GFP
    E Blank 488 / 10 SSC
    F Blank Blank N/A
    G Blank Blank Blank

    Red Trigon
    (633-nm red laser)

    A 755 LP 780 / 60 APC-Cy7
    B 685 LP 710 / 20 A680 ,
    APC-Cy5.5
    C Blank 660 / 20 APC , Cy5 , A647
    Violet Trigon
    (405-nm violet laser)
    A 505LP 525 / 50 CFP , Alexa 430
    B Blank 440 / 40 Alexa 405
    C ------ blank N/A
    UV Trigon
    (355-nm UV Laser)
    A 505LP 530 / 30

    Indo-1 (blue)

    B Blank 440 / 40 Hoechst 33342,
    DAPI , Alexa 350
    C ------ Blank N/A

Cell Sorters

  • MoFlo Astrios (2012)
  • FACSAriaII SORP (2010)
  • FACSVantage SE/Div (2002)
  • FACS Vantage SE/Diva

    Filter Configuration
    Excitation(nm) Fluorescenc Emission(nm) Fluorophore
    Argon Ion Laser
    488nm
    FL1 525 / 50 nm FITC , GFP
    FL2 575 / 26 nm PE
    FL3 622 / 36 nm PI
    FL6 667 / 30 nm PE-Cy5
    FL7 780 / 60 nm PE-Cy7
    FL8 710 / 50 nm PE-Cy5.5
    Dye Laser
    599nm
    FL4 670 / 10 nm APC , Cy5
    FL5 630 / 20 nm Texas Red
    FL9 780 / 60 nm APC-Cy7
    FL10 710 / 50 nm Alexa 680
    Krypton 302C Laser
    (uv351 nm)
    FL11 545 / 80 nm Indo-1 (blue)
    FL12 440 / 40 nm Hoechst 33342

Assistants

Ya-Min Lin
N604
TEL: 886-2-2789-9180
Mail: mbyamin@ccvax.sinica.edu.tw