Chen, Rey-Huei
Cell division cycle, mitosis
Cell division cycle is one of the most fundamental processes for all eukaryotic cells. It is orchestrated with precision to maintain genome stability and cell identity. The transitions of cell cycle stages are controlled by kinases, phosphatases and selective protein degradation, as well as checkpoint mechanisms that monitor the completion of various tasks in each stage. We study various aspects of the mitotic cell cycle mainly in budding yeast, using a combination of genetics, biochemistry and cell imaging. In recent years, we have focused on the essential function of Cdc48 chaperone in the cell cycle and the dynamic interaction between chromatin and the nuclear envelope. We are currently developing new interest in lipid homeostasis during the cell cycle.
Selected Recent Publications:
- Hsu, T.-H., Chen, R.-H., Cheng, Y.-H., and Wang, C.-W. 2017. Lipid droplets are central organelles for meiosis II progression during yeast sporulation. Mol. Biol. Cell 28(3): 440-451.
- Yang, P.-L., Hsu, T.-H., Wang, C.-W., and Chen, R.-H. 2016. Lipid droplets maintain lipid homeostasis during anaphase for efficient cell separation in budding yeast. Mol. Biol. Cell 27(15):2368-2380.
- Cheng, Y.-L. and Chen, R.-H. 2015. Assembly and quality control of the protein phosphatase 1 holoenzyme involves the Cdc48-Shp1 chaperone. J. Cell Sci. 128(6):1180-1192.
- Madsen, L., Molbæk, K., Larsen, I.B., Nielsen, S.V., Poulsen, E.G., Walmod, P.S., Hofmann, K., Seeger, M., Chien, C.Y., Chen, R.-H., Kriegenburg, F., Hartmann-Petersen, R. 2014. Human ASPL/TUG interacts with p97 and complements the proteasome mislocalization of a yeast ubx4 mutant, but not the ER-associated degradation defect. BMC Cell Biol. 15:31.
- Chien, C.-Y. and Chen, R.-H. 2013. Cdc48 chaperone and adaptor Ubx4 distribute the proteasome in the nucleus for anaphase proteolysis. J. Biol. Chem. 288(52): 37180-37191.
- Chen, R.-H. 2012. Nuclear envelope assembly and disassembly during the cell cycle. eLS. John Wiley & Sons, Ltd: Chichester.
- Tseng, L.-C. and Chen, R.-H. 2011. Temporal control of nuclear envelope assembly by phosphorylation of lamin B receptor. Mol. Biol. Cell 22:3306-3317.
- Hsieh, M.-T. and Chen, R.-H. 2011. Cdc48 and cofactors Npl4-Ufd1 are important for G1 progression during heat stress by maintaining cell wall integrity in Saccharomyces cerevisiae. PLoS ONE 6: e18988.
- Cheng, Y.-L. and Chen, R.-H. 2010. AAA-ATPase Cdc48 and Cofactor Shp1 promote chromosome bi-orientation by balancing Aurora B activity. J. Cell. Sci. 123: 2025-2034.