• Research News
• Research
• Faculty
• Core Facilities

Faculty Search:

Research News

Amino Acid Proximities in Two Sup35 Prion Strains Revealed by Chemical Cross-Linking

Chemical cross-linking and mass spectrometry are used to reveal how a single protein folds differently to form distinct prion strains. Intra- and intermolecular cross-linking are distinguished by MS/MS on the VK and VL strains seeded from equally mixed 14N- and 15N-labeled Sup35 protein. Amino acid residue adjacencies revealed by cross-linking are used to constrain different Sup35 chain folds.