Methods
Ⅰ DNA Microarray preparation |
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| A | DNA preparation for spotting — | Oligo (70 mer, 25 mer miRNA) cDNA ORF |
| B | Poly-L-lysine coated slides preparation | |
| C | Array design,printing and quality control | |
Ⅱ Sample preparation |
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| A | RNA isolation and purification | |
| B | RNA quality control | |
Ⅲ Target Labeling |
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| A | Direct incorporation | |
| 1. Total RNA direct labeling [Complement] [Short] | ||
| 2. Total RNA RT labeling check [Complement] [Short] | ||
| 3. gDNA labeling [Complement] [Short] | ||
| 4. small RNA labeling [Short] | ||
| B | Amino allyl-dUTP incorporation | |
| 1. Total RNA indirect-labeling [Complement] [Short] | ||
| 2. mRNA indirect-labeling [Complement] [Short] | ||
| 3. E.coli RNA indirect-labeling [Complement] [Short] | ||
| C | Secondary strand cDNA incorporation | |
| 2nd cDNA labeling [Complement] [Short] | ||
| D | Agilent low input amp and labeling [one color] | |
| E | 3D labeling ( Genisphere ) | |
| 3DNA array detection | ||
| F | Chemical labeling (KREATCH) | |
| 1. ULS small RNA labeling | ||
| 2. ULS aRNA fluorescent labeling | ||
Ⅳ Hybridization and wash |
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| A | Oligonucleotide Arrays on codelink activated slides [MAUI FL ] [MAUI DC] | |
| B | cDNA Arrays on Corning GAPSII Slides [GAPSII] | |
| C | Agilent [Gene Expression ] [aCGH ] [MAUI A4] [Agilent hybridizer-cRNA] [Agilent hybridizer-cDNA] | |
| D | NimbleGen [Gene expression ] [CHIP ] [Methylation ] | |
| E | Phalanx [MAUI FL] | |
Ⅴ Scanning |
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| A | Agilent | |
| B | Axon | |
| C | TECAN | |
Ⅵ Data Analysis |
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| A | GeneSpring (GX) | |
| B | SAM | |
| C | SMD | |