Hsou-min Li Lab

Dr. Hsou-min Li 李秀敏 博士
Distinguished Research Fellow, IMB

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研究方向

蛋白質運輸進入葉綠體的分子機制

綠色植物吸收陽光、二氧化碳與水，轉化成為澱粉，並釋放出氧氣，此光合作用乃萬物生命之所繫。而植物能進行光合作用的關鍵，在於細胞內的葉綠體必須輸入許多蛋白質。大多數的葉綠體蛋白質是在細胞質中合成，然後送入葉綠體。這些蛋白質能否被正確地送入葉綠體乃是決定了葉綠體能否正常運作。我們從結構及演化的角度，用遺傳及生化的方法，研究蛋白質輸入葉綠體的分子機制。藉由研究各個運輸機組成員之間的組合與交互作用，我們正逐步呈現蛋白質進入葉綠體的連續步驟。我們找到了聯接內外膜運輸機組的橋樑。我們也發現蛋白質進入葉綠體乃受植物發育及組織細胞調控。我們首先分離到一調控運輸機組基因在葉子表現的轉錄因子，又進一步發現蛋白質進入葉綠體會受植物年紀調控 ， 不同蛋白質喜好進入不同成長階段葉綠體，並找到一個蛋白質進入年老葉綠體及根部白色體的必要導引訊息，此訊息也是葉綠體導引訊息中第一個被找到的功能性序列。

 

 

Pictures from some of our papers

2001 images 
CIA2 is localized in the nucleus and responsible for the leaf-specific up-regulation of atTOC33 and atTOC75.
Sun,C.-W., Chen,L.-J., Lin,L.-C. and Li,H.-m*. (2001) Leaf-specific up-regulation of chloroplast translocon genes by a CCT-motif containing protein, CIA2. Plant Cell 13: 2053-2061.		 2001 images
A toc159 mutant does not have functional chloroplasts in mesophyll cells but has normal photosynthesizing/starch-accumulating chloroplasts in guard cells of stomata. Note the pairs of kidney-shaped guard cells.
Yu,T.-S. and Li,H.-m*. (2001) Chloroplast protein translocon components atToc159 and atToc33 are not essential for chloroplast biogenesis in guard cells and root cells. Plant Physiol. 127: 90-96.
2002 images
Crystal structure of pea Toc34 dimer
Sun,Y.-J., Forouhar,F., Li,H.-m., Tu,S.-L., Yeh,Y.-H., Kao,S., Shr,H.-L., Chou,C.-C., Chen, C. and Hsiao, C.D.* (2002) Crystal structure of pea Toc34 - a novel GTPase of the chloroplast protein translocon. Nature Structure Biology 9, 95-100.		 2007-01
Crystal structure of atToc33R130A, a mutant that can only form monomers.
Yeh, Y.-H., Kesavulu, M. M., Li, H.-m., Wu, S.-Z., Sun, Y.-J., Konozy, E. H. E. and Hsiao, C.-D.* (2007) Dimerization is important for the GTPase activity of chloroplast translocon components atToc33 and psToc159. J. Biol. Chem. 282: 13845-13853
2006-01
The cia5(tic21) mutant chloroplasts are specifically defective in protein translocation across the chloroplast envelope.
2006-02
Mutants of the two inner membrane channel components Tic20 and CIA5 (Tic21) and their double mutant.
Teng, Y.-S., Su, Y.-s., Chen, L.-J., Lee, Y.-J., Hwang, I. and Li, H.-m.* (2006) Tic21 is an essential translocon component for protein translocation across the chloroplast inner envelope membrane. Plant Cell 18, 2247-2257. 		2004-01
glutamine PRPP amidotranferase 1 is localized in chloroplasts
2004-02
Leaves of glutamine PRPP amidotranferase 2 mutants
Hung,W.-F., Chen,L.-J., Boldt,R., Sun,C.-w., and Li,H.-m.* (2004) Characterization of Arabidopsis glutamine PRPP amidotranferase-deficient mutants. Plant Physiol. 135, 1314-1323.
2009-1
cs215 is a dominant negative mutation in the CHLI1 gene.
Huang, Y.-S. and Li, H.-m.* (2009) Arabidopsis CHLI2 can substitute for CHLI1. Plant Physiol. 150:636-645.		 2010-1
cpHsc70s and Tic40 have a common vital function.
Su, P.-H. and Li, H.-m.* (2010) Stromal Hsp70 is important for protein translocation into pea and Arabidopsis chloroplasts. Plant Cell 22:1516-1531.
2012-01
Chloroplasts in leaves of different ages prefer to import different proteins
Teng, Y.-S., Chan, P.-T. and Li, H.-m.* (2012) Differential age-dependent import regulation by signal peptides. PLoS Biology 10:e1001416. 		2013-01
Li, H.-m.* and Teng, Y.-S. (2013) Transit peptide design and plastid import regulation. Trends Plant Sci. 18: 360-366.
2013-02
Tsai, J.-Y., Chu, C.-C., Yeh, Y.-H., Chen, L,-J., Li, H.-m.* and Hsiao, C-D.* (2013) Structural characterizations of chloroplast translocon protein Tic110. Plant J. 75:847-857.		 2016-01
Lin, Y.-T., Chen, L.-J., Herrfurth, C. Ivo Feussner, I. and Li, H.-m.* (2016) Reduced biosynthesis of digalactosyldiacylglycerol, a major chloroplast membrane lipid, leads to oxylipin overproduction and phloem cap lignification in Arabidopsis. Plant Cell, 28:219-232.
2016-02
Chen, Y.-L., Chen, L.-J. and Li, H.-m.* (2016) Polypeptide transport-associated domains of the Toc75 channel protein are located in the intermembrane space of chloroplast. Plant Physiol. 172: 235-243. DOI:10.1104/pp.16.00919.		 2019-02
Chen, Y.-L., Chen, L.-J., Chu, C.-C., Huang, P.-K., Wen, J.-R. and Li, H.-m.* (2018) TIC236 links the outer and inner membrane translocons of the chloroplast. Nature 564: 125-129. DOI: 10.1038/s41586-018-0713-y
2020
Tissue-Specific Regulation of Plastid Protein Import via Transit-Peptide Motifs. Chiung-Chih Chu, Krishna Swamy, and Hsou-min Li The Plant Cell 32: 1204–1217, 2020		 2022
TIC236 gain-of-function mutations unveil the link between plastid division and plastid protein import. Jun Fang, Bingqi Li, Lih-Jen Chen, Vivek Dogra, Shengji Luo, Wangpin Wu, Pengcheng Wang, Inhwan Hwang, Hsou-min Li* and Chanhong Kim* PNAS 119: e2123353119, 2022