ref: TruSeq DNA PCR-Free Sample Preparation Data Sheet TruSeq DNA PCR-Free Sample Preparation Guide
step | view | QC | Instrument or technology |
---|---|---|---|
Double stranded DNA | Gel/TapeStation NanoDrop Qubit |
||
Fragment DNA | Covaris/ bioanalyzer | ||
Library pre -repair End/ -select library size |
Bioanalyzer Ampure |
||
Library pre -adenylate 3' Ends -Ligate Adapters |
Qubit bioanalyzer |
||
Library preparation -measure conc |
Qubit qPCR |
||
Normalize and pool libraries |
Ref: truseq-chip-sample-prep-guide-15023092-b
16S ribosomal RNA (rRNA) sequencing is a common amplicon sequencing method used to identify and compare bacteria present within a given sample. 16S rRNA gene sequencing is a well-established method for studying phylogeny and taxonomy of samples from complex microbiomes or environments that are difficult or impossible to study.
Ref: 16s-metagenomic-library-prep-guide-15044223-b
Ref: Identify and compare bacteria from complex microbiomes or environments
Ref: Illumina 16S Metagenomic Sequencing Protocol FAQ
RNA sequencing
• mRNA-Seq (TruSeq Stranded mRNA Library Preparation)
• Total RNA seq (TruSeq Stranded Total RNA with Ribo-Zero)
• miRNA seq
• Targeted RNA Sequencing
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