purity | RNA/DNA Integrity description | |
---|---|---|
RNA | O.D.260/280: 1.9-2.1 O.D.260/230: 1.8-2.1 |
Bioanalyzer2100: 1. RIN ≥8.0, 2. rRNA% ≥40%, 3. 18s/28s ≥1.8, 4. gDNA: Non-detected (DNase treated) |
DNA | O.D.260/280: 1.8-2.1 O.D.260/230: 1.8-2.1 |
Sample 在gel picture上無明顯smear (RNase A treated-phenol:chloroform purify) |
Bioanalyzer 2100 | Qty | Quantitative range | Q-bit | Quantitative range |
---|---|---|---|---|
RNA 6000 Nano | 12 | 25-500ng/ul(200ng/ul) | BR RNA assay | 20-1000ng |
RNA 6000 pico | 11 | 50-5000pg/ul | RNA assay | 5-100ng |
DNA 7500 (100-7500bp) | 12 | 0.5-50ng/ul | dsDNA BR assay | 2-1000ng |
DNA HS (50-7000bp) | 11 | 5-500pg/ul | dsDNA HS assay | 0.2-100ng |
* Please give us >2ul of each RNA/DNA sample in 0.5 tubes. |
Application | Nucleic acid* quantity |
Conc. | buffer recommendations |
Note | |
---|---|---|---|---|---|
Gene expression | Recommend | 10-15ug | ≥1ug/ul | Nuclease free water | Do not containment genomic DNA |
Amplication | 200-500 ng | ||||
miRNA | totalRNA | 5ug | ≥100ng | Nuclease free water | |
enrichedRNA | 500ng | ≥50ng/ul | |||
aCGH | 2ug | ≥80ng/ul | TE / Nuclease free water | ||
ChIP-chip | 2ug | ≥80ng/ul | TE/Nuclease free water | DNA fragment 200bp-1Kb (major at 500bp) | |
* base on fluorometric quantification by Qubit assay |
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