The Genomics Core hosts four platforms for determination of sample quality and integrity for both DNA and RNA samples.
Measure the concentration and purity of your DNA, RNA samples using the Thermo Scientific NanoDrop 2000 spectrophotometer and be confident in your downstream application results. Using only 1 µL, you can obtain your results in less than 15 seconds from sample pipetting to wiping the pedestal clean.
Based on a simple mix-and-read format, Invitrogen™ Qubit™ assays can be read in the Qubit Fluorometer following as little as two minutes' incubation. The dye in each Qubit Assay Kit emits fluorescence only when specifically bound to the target molecule, which minimizes the effects of contaminants—including degraded DNA or RNA—on the result. You can use the fellow Qubit assay kit in our Core.
Qubit assay kit | Quantitation range | Applications |
---|---|---|
Qubit dsDNA BR Assay Kit | 2–1,000 ng | Quantitation of genomic and miniprep DNA samples |
Qubit dsDNA HS Assay Kit | 0.2–100 ng | Quantitation of PCR products, viral DNA, or samples for NGS |
Qubit RNA BR Assay Kit | 20–1,000 ng | Quantitation of samples for microarray, RT-PCR, and northern blot procedures |
Qubit RNA HS Assay Kit | 5–100 ng | |
Qubit ssDNA Assay Kit | 1–200 ng | Useful for quantitation of oligos, primers, denatured DNA, PCR products |
The Agilent 2100 Bioanalyzer system provides sizing, quantitation and quality control of DNA, RNA, proteins and cells on a single platform, providing high quality digital data. We provide the following assay kit service.
Note: Please make an appointment the day before this service. Please give us >2uL of each sample in 0.5 tubes
Kit | Qty/chip | Sizing range | Quantitative range |
---|---|---|---|
RNA 6000 Nano | 12 | 25-500 ng/mL (200ng/uL) | |
RNA 6000 pico | 11 | 0.05-5 ng/uL | |
DNA High Sensitivity | 11 | 50-7000 bp | 5-5000 pg/uL |
The Agilent 2200 TapeStation system automates RNA, DNA and protein sample QC, including sample loading, separation, and imagin. You can use the following kit in our Core.
Kit | Qty/chip | Sizing range | Quantitative range |
---|---|---|---|
RNA Screen Tape | 2n | 100-6000nt | 25-500 ng/uL |
High Sensitivity RNA | 2n | 100-6000nt | 500-10000pg/uL |
High Sensitivity D1000 | 2n | 35bp-1kb | 0.1-1 ng/uL |
Genomic DNA assay | 2n | 200bp-60kb | 10-100 ng/uL |
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