1. Cell sorting

• High speed 4-way sorting
• 96-well single cell sorting
• Routine checking of sterile sorting

2. Maintenance of FACS Machines

• Daily instrument quality control
• Laser alignment

3. Training Courses for FACS Users

• Principles of FACS
• Operation of flow cytometer
• Multiple color data acquisition and analysis
• DNA cell cycle data acquisition and analysis

4. Troubleshooting

分生所同仁預約使用

Cell Sorting	1. Isolation of cell sub-populations
	2. Selection of transgene-expressing cells
	3. Cloning of hybridomas or cell lines
Cell Analysis	1. Analysis of cell surface markers
	2. Analysis of intracellular cytokine production
	3. Monitoring of intracellular Ca2+ mobilization
	4. Determination of cell death
	5. Measurement of cell cycle status