真菌與線蟲的分子交互作用及共同演化

線蟲是地球上數量最多的動物。據估計，地球表層土壤中存在約有 4 x 1020 的線蟲，而許多線蟲是寄生性線蟲，造成人類，動物，及植物的各種疾病。全球估計有超過十億人口受到寄生蟲的感染，而在農業上，每年因寄生性線蟲在各種作物造成的經濟損失則高達 800億美金。 目前人類可以使用的抗寄生蟲藥物種類並不多，以伊維菌素（Ivermectin）最為普遍。近年來伊維菌素抗藥性問題日趨嚴重，因此我們急需發展新型抗寄生蟲藥物或是新的方法，來防治寄生性線蟲。 在自然界中，線蟲存在於復雜的群落中，依靠著精細的化學信號網絡與其他物種產生交互作用，其中亦包括了線蟲的天敵，食線蟲真菌。許多不同的真菌例如線蟲捕捉菌及杏鮑菇，秀珍菇，蠔菇等側耳屬菇類在演化的過程中，獨立演化出捕食線蟲的能力，從線蟲獲取養分。 食蟲真菌和線蟲之間的天敵關係使它們成為研究種間交互作用和掠食者/ 獵物之間共同演化的理想系統。本實驗室建立了線蟲捕捉菌及蠔菇兩種非模式生物的分子遺傳系統，並利用秀麗隱桿線蟲來系統性的研究肉食性真菌獵食，毒殺線蟲的分子機制及掠食者/獵物之間共同演化。 本實驗室採用的系統性方法包含遺傳學，基因體學，分子生物學，生物化學，影像分析，細胞生物學，神經科學，及化學分析等，聚焦回答以下幾個主要問題：

1. 線蟲捕捉菌偵測線蟲費洛蒙，發育捕捉構造的分子機制
2. 線蟲捕捉菌黏著，侵染線蟲獵物的的分子機制
3. 線蟲捕捉菌捕食線蟲能力的演化
4. 蠔菇毒殺線蟲成分的分析
5. 蠔菇發育特殊毒殺線蟲構造的分子機制
6. 秀麗隱桿線蟲偵測掠食者的神經分子機制
7. 秀麗隱桿線蟲對掠食者的行為演化

• Education
• Achievements & Honors
• Selected publications
• Lab members

• PDF, 2010-2015, Division of Biology and Biological Engineering, California Institute of Technology
• Ph.D., 2008-2010, Department of Molecular Genetics and Microbiology, Duke University
• MS, 2003, Department of Plant Pathology and Microbiology, National Taiwan University
• BS, 2001, Department of Plant Pathology and Microbiology, National Taiwan University

• 2022, 中央研究院年輕學者研究成果獎
• 2022, 台灣傑出女科學家新秀獎
• 2021, 李天德青年醫藥科技獎
• 2020, 歐洲分子生物學組織年輕學者
• 2019, 歐洲分子生物學組織全球學者
• 2018, 傑出人才基金會年輕學者創新獎
• 2014, NIH/NIGMS Pathway to Independence (PI) K99 Award
• 2008, Genetics Society of America, DeLill Nasser Award

1. Ching-Han Lee, Yi-Yun Lee, Yu-Chu Chang, Wen-Li Pon, Sue-Ping Lee, Niaz Wali, Takehito Nakazawa, Yoichi Honda, Jiun-Jie Shie, Yen-Ping Hsueh (2023) A carnivorous mushroom paralyzes and kills nematodes via a volatile ketone. SCIENCE ADVANCES 18 Jan 2023 Vol 9, Issue 3.
2. Sheng-An Chen, Hung-Che Lin, Yen-Ping Hsueh (2022) The cAMP-PKA pathway regulates prey sensing and trap morphogenesis in the nematode-trapping fungus Arthrobotrys oligospora. G3 Genes|Genomes|Genetics 12, jkac217 (Cover)
3. Sheng-An Chen, Hung-Che Lin, Frank C Schroeder, Yen-Ping Hsueh (2021) Prey sensing and response in a nematodetrapping fungus is governed by the MAPK pheromone response pathway. Genetics 217: jyaa008.
4. Tsung-Yu Huang, Yi-Yun Lee, Guillermo Vidal-Diez de Ulzurrun, Yen-Ping Hsueh (2021) Forward genetic screens identified mutants with defects in trap morphogenesis in the nematode-trapping fungus Arthrobotrys oligospora. G3 11: jkaa022.
5. Yi-Yun Lee, Guillermo Vidal-Diez de Ulzurrun, Erich M Schwarz, Jason E Stajich, Yen-Ping Hsueh (2021) Genome sequence of the oyster mushroom Pleurotus ostreatus strain PC9. G3 11: jkaa008.
6. Ching-Ting Yang, Guillermo Vidal-Diez de Ulzurrun, A Pedro Gonçalves, Hung-Che Lin, Ching-Wen Chang, Tsung-Yu Huang, Sheng-An Chen, Cheng-Kuo Lai, Isheng J Tsai, Frank C Schroeder, Jason E Stajich, Yen-Ping Hsueh (2020) Natural diversity in the predatory behavior facilitates the establishment of a robust model strain for nematode- trapping fungi. PNAS 117: 6762-6770.
7. Ching-Han Lee, Han-Wen Chang, Ching-Ting Yang, Niaz Wali, Jiun-Jie Shie, Yen-Ping Hsueh (2020) Sensory cilia as the Achilles heel of nematodes when attacked by carnivorous mushrooms. PNAS 117: 6014-6022.
8. Guillermo Vidal-Diez de Ulzurrun, Tsung-Yu Huang, Ching-Wen Chang, Hung-Che Lin, Yen-Ping Hsueh (2019) Fungal feature tracker (FFT): A tool for quantitatively characterizing the morphology and growth of filamentous fungi. PLoS Comput Biol. 15: e1007428.
9. Yen-Ping Hsueh, Matthew R Gronquist, Erich M Schwarz, Ravi David Nath, Ching-Han Lee, Shalha Gharib, Frank C Schroeder, Paul W Sternberg (2017) Nematophagous fungus Arthrobotrys oligospora mimics olfactory cues of sex and food to lure its nematode prey. eLife 6: e20023.