Membrane fluidity, lipid homeostasis, proteostasis, organelle dynamics

脂質為細胞膜、能源和信號分子的結構成分。具流動性 的細胞膜讓膜融合和分裂、內吞和胞吐、細胞分裂過程 中的細胞器分離、以及膜內蛋白質移動，這類和胞膜有 關的動態現象可以發生。膜的流動性多取決於不飽和 脂質和固醇的量，因其控制膜中脂質排列的規律與緻密 度。碳水化合物鏈中具有雙鍵的不飽和脂肪酸對於生命 至關重要。然而，我們卻不瞭解脂質飽和度如何影響細 胞膜的功能和動態。我們用出芽酵母菌這個模式生物來 探討這個問題，因為它含有單一個脂肪酸去飽和酶基因 OLE1，所以它比其他系統更具研究上的優勢。我們已 經分離出對溫度敏感的ole1 突變體，並用遺傳，生化， 細胞生物學和系統分析等方法來研究不飽和脂肪酸的細 胞功能。我們目前的研究重點包括：

1. 脂質飽和度對整體細胞膜結構和動態的影響
2. 不飽和脂肪酸和固醇合成彼此間的交互調節，以及其如何維持膜穩態
3. 脂質飽和對膜蛋白折疊的影響，以及細胞如何應對此改變

我們的目標是最終從分子細節上了解脂質飽和度的變異 如何被感知並傳遞給脂質穩態和蛋白穩態的調節，以及 該過程如何影響細胞生理。

• Education
• Achievements & Honors
• Selected publications
• Lab members

• PDF, 1994-1997, University of California, San Francisco USA
• Ph.D., 1993, Department of Cell. & Mol. Physiology Harvard University, USA
• D.D.S., 1987, Department of Dentistry National Taiwan University

• 2005-2009, Academia Sinica Investigator Award
• 1998-2003, David and Lucile Packard Award in Science and Engineering, USA
• 1994-1997, Helen Hay Whitney Postdoctoral Research Fellowship, USA

1. Huang, L.-J. and Chen, R.-H. 2022. Lipid saturation induces degradation of squalene epoxidase for sterol homeostasis and cell survival. Life Sci. Alliance 6(1):e202201612.
2. Chen, R.-H. 2019. Chromosome detachment from the nuclear envelope is required for genome stability in closed mitosis. Mol. Biol. Cell 30(13):1578-1586.
3. Hsu, T.-H., Chen, R.-H., Cheng, Y.-H., and Wang, C.-W. 2017. Lipid droplets are central organelles for meiosis II progression during yeast sporulation. Mol. Biol. Cell 28(3): 440-451.
4. Yang, P.-L., Hsu, T.-H., Wang, C.-W., and Chen, R.-H. 2016. Lipid droplets maintain lipid homeostasis during anaphase for efficient cell separation in budding yeast. Mol. Biol. Cell 27(15):2368-2380.
5. Cheng, Y.-L. and Chen, R.-H. 2015. Assembly and quality control of the protein phosphatase 1 holoenzyme involves the Cdc48-Shp1 chaperone. J. Cell Sci. 128(6):1180-1192.
6. Madsen, L., Molbæk, K., Larsen, I.B., Nielsen, S.V., Poulsen, E.G., Walmod, P.S., Hofmann, K., Seeger, M., Chien, C.Y., Chen, R.-H., Kriegenburg, F., Hartmann-Petersen, R. 2014. Human ASPL/TUG interacts with p97 and complements the proteasome mislocalization of a yeast ubx4 mutant, but not the ER-associated degradation defect. BMC Cell Biol. 15:31.
7. Chien, C.-Y. and Chen, R.-H. 2013. Cdc48 chaperone and adaptor Ubx4 distribute the proteasome in the nucleus for anaphase proteolysis. J. Biol. Chem. 288(52): 37180-37191.
8. Chen, R.-H. 2012. Nuclear envelope assembly and disassembly during the cell cycle. eLS. John Wiley & Sons, Ltd: Chichester.
9. Tseng, L.-C. and Chen, R.-H. 2011. Temporal control of nuclear envelope assembly by phosphorylation of lamin B receptor. Mol. Biol. Cell 22:3306-3317.
10. Hsieh, M.-T. and Chen, R.-H. 2011. Cdc48 and cofactors Npl4-Ufd1 are important for G1 progression during heat stress by maintaining cell wall integrity in Saccharomyces cerevisiae. PLoS ONE 6: e18988.
11. Cheng, Y.-L. and Chen, R.-H. 2010. AAA-ATPase Cdc48 and Cofactor Shp1 promote chromosome bi-orientation by balancing Aurora B activity. J. Cell. Sci. 123: 2025-2034.